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	Provedor de dados ArchiMer (3) Autor Michel, Justine (3) Arzul, Isabelle (2) Chollet, Bruno (2) Francois, Cyrille (2) Garcia, Celine (2) Miossec, Laurence (2) Robert, Maeva (2) Joly, Jean-pierre (1) Mais... Palavra-chave Parasite (2) Perkinsus olseni (2) Ruditapes decussatus (2) Ruditapes philippinarum (2) Actin (1) Clam parasites (1) Clams (1) French producing areas (1) ISH (1) PCR (1) Perkinsus (1) Perkinsus chesapeaki (1) Ribosomal RNA (1) Variabilité génétique (1) Mais... Tipo do documento Text (3) Ano 2012 (1) 2009 (1) 2008 (1) País France (3) Idioma Inglês (2) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Caractérisation moléculaire du parasite du genre Perkinsus infectant les palourdes en France et étude du polymorphisme génétique en fonction de son origine géographique ArchiMer Michel, Justine. En 2004 et 2005, une étude menée par le REPAMO (Réseau de Pathologie des Mollusques) a montré que des parasites du genre Perkinsus étaient présentas au sein des principaux domaines français de production de palourdes: La Mer Méditerranée, l'Océan Atlantique et la Manche avec une forte prévalence amis aucun cas de mortalités associées. Cependant, ces résultats doivent être complétés par des analyses moléculaires plus spécifiques de façon à déterminer la ou les espèces de Perkinsus détectés. D'autre, part, l'étude a montré que différents facteurs environnementaux (température, salinité...) liés à la répartition géographique influencent le développement de parasite. Ces résultats suggèrent l'existence d'une variabilité génétique chez le ou les parasites du... Tipo: Text Palavras-chave: Parasite; Perkinsus; PCR; Ruditapes decussatus; Ruditapes philippinarum; Variabilité génétique. Ano: 2008 URL: http://archimer.ifremer.fr/doc/00033/14453/11754.pdf Molecular characterization of parasites of the genus Perkinsus present in clams from French producing areas ArchiMer Arzul, Isabelle; Michel, Justine; Chollet, Bruno; Robert, Maeva; Miossec, Laurence; Garcia, Celine; Francois, Cyrille. Production of clams is the third most important bivalve production in France and was estimated at 3400 metric tons in 2001 among which 60% came from aquaculture and 40% from fisheries. Surveillance of clam diseases mainly relies on histology and thioglycolate culture, two techniques allowing detection of parasites of the genus Perkinsus but not specific enough to determine the parasite species. Based on a previous study aiming at estimating distribution and prevalence of perkinsosis in France, we selected 34 infected clams from 4 different producing areas in order to characterize the parasite species. Molecular characterization was done by PCR-RFLP targeting the ITS region as described by Abollo et al. (2006). 16 PCR products were cloned and 10 to 20... Tipo: Text Palavras-chave: French producing areas; Clams; Perkinsus olseni; Parasite. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/acte-6269.pdf One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France ArchiMer Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence. Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in... Tipo: Text Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf Registros recuperados: 3 Primeira ... 1 ... Última

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